Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 16-5899-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: This AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis and functional assays (such as stimulation of basophil migration). Applications Tested: This AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AER-37 (CRA1)
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Distinct parameters of the basophil activation test reflect the severity and threshold of allergic reactions to peanut.
Serum IgE clearance is facilitated by human FcεRI internalization.
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Fc-epsilon-RI, the high affinity IgE-receptor, is robustly expressed in the upper gastrointestinal tract and modulated by mucosal inflammation.
Early gene expression changes with rush immunotherapy.
Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum.
A soluble form of the high affinity IgE receptor, Fc-epsilon-RI, circulates in human serum.
Comparative analysis of FcεRI expression patterns in patients with eosinophilic and reflux esophagitis.
Anti-IgE treatment of eosinophil-associated gastrointestinal disorders.
IgE- and FcepsilonRI-mediated migration of human basophils.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Santos AF, Du Toit G, Douiri A, Radulovic S, Stephens A, Turcanu V, Lack G
The Journal of allergy and clinical immunology 2015 Jan;135(1):179-86
The Journal of allergy and clinical immunology 2015 Jan;135(1):179-86
Serum IgE clearance is facilitated by human FcεRI internalization.
Greer AM, Wu N, Putnam AL, Woodruff PG, Wolters P, Kinet JP, Shin JS
The Journal of clinical investigation 2014 Mar;124(3):1187-98
The Journal of clinical investigation 2014 Mar;124(3):1187-98
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Fc-epsilon-RI, the high affinity IgE-receptor, is robustly expressed in the upper gastrointestinal tract and modulated by mucosal inflammation.
Bannert C, Bidmon-Fliegenschnee B, Stary G, Hotzy F, Stift J, Nurko S, Szépfalusi Z, Fiebiger E, Dehlink E
PloS one 2012;7(7):e42066
PloS one 2012;7(7):e42066
Early gene expression changes with rush immunotherapy.
Davis LS, Bhutani S, Barnett SR, Khan DA
Clinical and molecular allergy : CMA 2011 Sep 30;9:12
Clinical and molecular allergy : CMA 2011 Sep 30;9:12
Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum.
Lexmond W, der Mee Jv, Ruiter F, Platzer B, Stary G, Yen EH, Dehlink E, Nurko S, Fiebiger E
Journal of immunological methods 2011 Oct 28;373(1-2):192-9
Journal of immunological methods 2011 Oct 28;373(1-2):192-9
A soluble form of the high affinity IgE receptor, Fc-epsilon-RI, circulates in human serum.
Dehlink E, Platzer B, Baker AH, Larosa J, Pardo M, Dwyer P, Yen EH, Szépfalusi Z, Nurko S, Fiebiger E
PloS one 2011 Apr 22;6(4):e19098
PloS one 2011 Apr 22;6(4):e19098
Comparative analysis of FcεRI expression patterns in patients with eosinophilic and reflux esophagitis.
Yen EH, Hornick JL, Dehlink E, Dokter M, Baker A, Fiebiger E, Nurko S
Journal of pediatric gastroenterology and nutrition 2010 Nov;51(5):584-92
Journal of pediatric gastroenterology and nutrition 2010 Nov;51(5):584-92
Anti-IgE treatment of eosinophil-associated gastrointestinal disorders.
Foroughi S, Foster B, Kim N, Bernardino LB, Scott LM, Hamilton RG, Metcalfe DD, Mannon PJ, Prussin C
The Journal of allergy and clinical immunology 2007 Sep;120(3):594-601
The Journal of allergy and clinical immunology 2007 Sep;120(3):594-601
IgE- and FcepsilonRI-mediated migration of human basophils.
Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M
International immunology 2005 Sep;17(9):1249-55
International immunology 2005 Sep;17(9):1249-55
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human lysed whole blood with Mouse IgG2b Isotype Control Purified (Product # 14-4732-82) (green histogram) or Anti-Human Fc epsilon Receptor I alpha (Fc epsilon R1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85)and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.