16-5899-82

antibody from Invitrogen Antibodies
Targeting: FCER1A FCE1A
Provider product page for 16-5899-82
Flow cytometry
Other assay

Antibody data

Product number
16-5899-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: This AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis and functional assays (such as stimulation of basophil migration). Applications Tested: This AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
AER-37 (CRA1)
Vial size
100 µg
Concentration
1 mg/mL
Storage
4° C
FCER1A protein structure - 16-5899-82 shown in red.