H00002205-M01

antibody from Novus Biologicals

Targeting: FCER1A FCE1A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00002205-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00002205-M01, RRID:AB_538782

Product name

Mouse Monoclonal Fc epsilon RI Antibody

Antibody type

Monoclonal

Description

IgG purified. FCER1A - Fc fragment of IgE, high affinity I, receptor for

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.Lane 1: FCER1A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - FCER1A monoclonal antibody (M01), clone 2C12-3B6 Analysis of FCER1A expression in A-549.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.