Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00002205-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002205-M01, RRID:AB_538782
- Product name
- Mouse Monoclonal Fc epsilon RI Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FCER1A - Fc fragment of IgE, high affinity I, receptor for
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.Lane 1: FCER1A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - FCER1A monoclonal antibody (M01), clone 2C12-3B6 Analysis of FCER1A expression in A-549.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.