AP17316b

antibody from Abgent

Targeting: STK11 LKB1, PJS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP17316b

Provider

Abgent

Proper citation

Abgent Cat#AP17316b, RRID:AB_11132786

Product name

Mouse Stk11 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Mouse Stk11 Antibody (C-term) (Cat. #AP17316b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Stk11 antibody detected the Stk11 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysate from NCI-H1299 cell line, using Mouse Stk11 Antibody (C-term)(Cat. #AP17316b). AP17316b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:500-1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody (C-term)(Cat#AP17316b). AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Primary Ab dilution

1:25

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Immunohistochemical analysis of paraffin-embedded M.skeletal muscle section using Mouse Stk11 Antibody (C-term)(Cat#AP17316b). AP17316b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining."

Primary Ab dilution

1:25