H00006794-D01P

antibody from Abnova Corporation

Targeting: STK11 LKB1, PJS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006794-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006794-D01P, RRID:AB_1580038

Product name

STK11 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human STK11 protein.

Antigen sequence

MEVVDPQQLGMFTEGELMSVGMDTFIHRIDSTEVI
YQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSE
TLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRL
RHKNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEML
DSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHK
DIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTC
RTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTL
YNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGP
PLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPP
AEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADED
EDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRG
LPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSA
SSKIRRLSACKQQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of STK11 expression in transfected 293T cell line (H00006794-T01) by STK11 MaxPab polyclonal antibody.Lane 1: STK11 transfected lysate(48.60 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab rabbit antibody to STK11 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between STK11 and PRKAA2. HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-PRKAA2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)