PA5-96062

antibody from Invitrogen Antibodies

Targeting: STK11 LKB1, PJS

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96062

Provider

Invitrogen Antibodies

Product name

LKB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: CGMQEMLDSV PEKRFPVCQA HGYFCQLIDG LEYLHSQGIV HKDIKPGNLL LTTGGTLKIS DLGVAEALHP FAADDTCRTS QGSPAFQPPE IANGLDTFSG FKVDIWSAGV TLYNITTGLY PFEGDNIYKL FENIGKGSYA IPGDCGPPLS DLLKGMLEYE PAKRFSIRQI RQHSWFRKKH PPAEAPVPIP PSPDTKDRWR SMTVVPYLED LHGADEDEDL FDIEDDIIYT QDFTVPGQVP EEEASHNGQR RGLPKAVCMN GTEAAQLSTK SRAEGRAPNP ARKACSASSK IRRLSACKQQ; Positive Samples: U-87MG, SKOV3, Mouse heart; Cellular Location: Cytoplasm, Membrane, Mitochondrion, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.75 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes.

He C, Gao P, Cui Y, Li Q, Li Y, Lu Z, Ma H, Zhao Y, Li L, Sun F, Chen X, Jia H, Liu D, Yang G, Zheng H, Zhu Z
Clinical and translational medicine 2020 Oct;10(6):e205

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of LKB1 was performed in MCF-7 cells using LKB1 Polyclonal Antibody (Product # PA5-96062). Blue: DAPI for nuclear staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6 Inhibition of AMPK by TRPC6/ CaMKK2 pathway disturbs phosphorylation of Drp1. (A) Representative Western blots of AMPKalpha and p-AMPKalpha in PC12 cells or mouse hippocampus. The quantitative data are showed on the right (n = 3 for PC12 cells, n = 4 tissues from four mice). (B) Representative Western blots of LKB1, CaMKK2, p-CaMKK2, AMPKalpha, and p-AMPKalpha in the hippocampus of DM mice with or without RH. The quantitative data are shown on the right (n = 3 tissues from three mice). TRPC6 -/- , TRPC6 global knockout mice. (C) Representative Western blots of LKB1, CaMKK2, p-CaMKK2, AMPKalpha, and p-AMPKalpha in the hippocampus of hyperforin or vehicle-treated diabetic WT or TRPC6 -/- -mice with RH. The quantitative data are showed on the right (n = 3). Hyp, hyperforin. (D) The protein expression level of AMPKalpha, p-AMPKalpha, Drp1, p-Drp1, (S616), and p-Drp1 (S637) in PC12 cells treated with CC. The statistical results are showed on the right (n = 3). CC, AMPK inhibitor compound C (10 uM); Lv-TRPC6, TRPC6 overexpression with lentivirus; Lv-Con, the vehicle lentivirus. (E) The hyperforin (10 uM) evoked intracellular Ca 2+ influx in PC12 cells cultured. The quantitative data are showed on the right (n = 3). TRPC6-mediated Ca 2+ influx was determined by the subtraction of hyperforin- and Ca 2+ -induced Ca 2+ influx to Ca 2+ -induced influx. Ca 2+ (1 mM) and/or hypforin (10 uM) was added at 25S. Ca 2+ , Cacl 2 (1 mM); Hyp, hyperforim (10 uM). (F) The protein levels o