Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-15729 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LKB1 Monoclonal Antibody (4H12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15729 targets STK11 in indirect ELISA, FACS and WB applications and shows reactivity with Human, mouse, and Non-human primate samples. The MA5-15729 immunogen is purified recombinant fragment of human STK11 expressed in E. Coli. . MA5-15729 detects STK11 which has a predicted molecular weight of approximately 54kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H12
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of LKB1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR870103_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of LKB1 was performed by loading 30 µg of A-431 wild type (Lane 1), A-431 CAS9 (Lane 2), A-431 LKB1 KO (Lane 3) whole cell extracts. The blot was probed with Anti-LKB1 Monoclonal Antibody (4H12)(Product # MA5-15729) using 1:1000 dilution and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to LKB1. An uncharacterized band was observed at ~30kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of LKB1 was achieved by transfecting A-431 with LKB1 specific siRNAs (Silencer® select Products # s532020, s501375). Western blot analysis (Fig. a) was performed using whole cell extracts from the LKB1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed withLKB1 Monoclonal Antibody (4H12) (Product # MA5-15729, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to LKB1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-LKB1 Monoclonal Antibody (4H12) (Product # MA5-15729) and a 55kDa band corresponding to LKB1 was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of MCF7 (Lane 1), A-431 (Lane 2) and U-2 OS (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076)..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STK11 using STK11 monoclonal antibody (Product # MA5-15729) in NIH/3T3 (1), Raw246.7 (2), COS-7 (3), Jurkat (4), HEK293 (5) and A431 (6) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells using STK11 monoclonal antibody (Product # MA5-15729) (green) and negative control (purple).