MA5-15729

antibody from Invitrogen Antibodies

Targeting: STK11 LKB1, PJS

ELISA

Antibody data

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Validation data

Product number: MA5-15729 - Provider product page
Provider: Invitrogen Antibodies
Product name: LKB1 Monoclonal Antibody (4H12)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15729 targets STK11 in indirect ELISA, FACS and WB applications and shows reactivity with Human, mouse, and Non-human primate samples. The MA5-15729 immunogen is purified recombinant fragment of human STK11 expressed in E. Coli. . MA5-15729 detects STK11 which has a predicted molecular weight of approximately 54kDa.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 4H12
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

STK11 protein structure - MA5-15729 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of LKB1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR870103_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of LKB1 was performed by loading 30 µg of A-431 wild type (Lane 1), A-431 CAS9 (Lane 2), A-431 LKB1 KO (Lane 3) whole cell extracts. The blot was probed with Anti-LKB1 Monoclonal Antibody (4H12)(Product # MA5-15729) using 1:1000 dilution and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to LKB1. An uncharacterized band was observed at ~30kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of LKB1 was achieved by transfecting A-431 with LKB1 specific siRNAs (Silencer® select Products # s532020, s501375). Western blot analysis (Fig. a) was performed using whole cell extracts from the LKB1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed withLKB1 Monoclonal Antibody (4H12) (Product # MA5-15729, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to LKB1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-LKB1 Monoclonal Antibody (4H12) (Product # MA5-15729) and a 55kDa band corresponding to LKB1 was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of MCF7 (Lane 1), A-431 (Lane 2) and U-2 OS (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076)..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of STK11 using STK11 monoclonal antibody (Product # MA5-15729) in NIH/3T3 (1), Raw246.7 (2), COS-7 (3), Jurkat (4), HEK293 (5) and A431 (6) cell lysate.