44-836G

antibody from Invitrogen Antibodies

Targeting: PEA15 HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED, PED-PEA15, PED/PEA15

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

44-836G

Provider

Invitrogen Antibodies

Product name

Phospho-PEA15 (Ser116) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C

References

Submitted references

Integrated Molecular Characterization of Uterine Carcinosarcoma.

Cherniack AD, Shen H, Walter V, Stewart C, Murray BA, Bowlby R, Hu X, Ling S, Soslow RA, Broaddus RR, Zuna RE, Robertson G, Laird PW, Kucherlapati R, Mills GB, Cancer Genome Atlas Research Network, Weinstein JN, Zhang J, Akbani R, Levine DA
Cancer cell 2017 Mar 13;31(3):411-423

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Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling.

Hill SM, Nesser NK, Johnson-Camacho K, Jeffress M, Johnson A, Boniface C, Spencer SE, Lu Y, Heiser LM, Lawrence Y, Pande NT, Korkola JE, Gray JW, Mills GB, Mukherjee S, Spellman PT
Cell systems 2017 Jan 25;4(1):73-83.e10

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The protein phosphatase 4 - PEA15 axis regulates the survival of breast cancer cells.

Mohammed HN, Pickard MR, Mourtada-Maarabouni M
Cellular signalling 2016 Sep;28(9):1389-1400

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Effects of anti-depressant treatments on FADD and p-FADD protein in rat brain cortex: enhanced anti-apoptotic p-FADD/FADD ratio after chronic desipramine and fluoxetine administration.

García-Fuster MJ, García-Sevilla JA
Psychopharmacology 2016 Aug;233(15-16):2955-71

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The neuroplastic index p-FADD/FADD and phosphoprotein PEA-15, interacting at GABAA receptor, are upregulated in brain cortex during midazolam-induced hypnosis in mice.

Álvaro-Bartolomé M, García-Sevilla JA
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 2015 Nov;25(11):2131-44

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Regulation of hippocampal Fas receptor and death-inducing signaling complex after kainic acid treatment in mice.

Keller B, García-Sevilla JA
Progress in neuro-psychopharmacology & biological psychiatry 2015 Dec 3;63:54-62

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FADD adaptor and PEA-15/ERK1/2 partners in major depression and schizophrenia postmortem brains: basal contents and effects of psychotropic treatments.

García-Fuster MJ, Díez-Alarcia R, Ferrer-Alcón M, La Harpe R, Meana JJ, García-Sevilla JA
Neuroscience 2014 Sep 26;277:541-51

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CDKN2D-WDFY2 is a cancer-specific fusion gene recurrent in high-grade serous ovarian carcinoma.

Kannan K, Coarfa C, Rajapakshe K, Hawkins SM, Matzuk MM, Milosavljevic A, Yen L
PLoS genetics 2014 Mar;10(3):e1004216

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Molecular adaptations of apoptotic pathways and signaling partners in the cerebral cortex of human cocaine addicts and cocaine-treated rats.

Alvaro-Bartolomé M, La Harpe R, Callado LF, Meana JJ, García-Sevilla JA
Neuroscience 2011 Nov 24;196:1-15

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The time course of unconditioned morphine-induced psychomotor sensitization mirrors the phosphorylation of FADD and MEK/ERK in rat striatum: role of PEA-15 as a FADD-ERK binding partner in striatal plasticity.

Ramos-Miguel A, Esteban S, García-Sevilla JA
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 2010 Jan;20(1):49-64

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Protein phosphatase 4 regulates apoptosis, proliferation and mutation rate of human cells.

Mourtada-Maarabouni M, Williams GT
Biochimica et biophysica acta 2008 Aug;1783(8):1490-502

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Phosphorylation of PEA-15 switches its binding specificity from ERK/MAPK to FADD.

Renganathan H, Vaidyanathan H, Knapinska A, Ramos JW
The Biochemical journal 2005 Sep 15;390(Pt 3):729-35

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Phosphorylation of phosphoprotein enriched in astrocytes (PEA-15) regulates extracellular signal-regulated kinase-dependent transcription and cell proliferation.

Krueger J, Chou FL, Glading A, Schaefer E, Ginsberg MH
Molecular biology of the cell 2005 Aug;16(8):3552-61

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lysates of PMA-stimulated Cos7 cells transfected with HA-tagged wild-type PED/PEA-15 (A), His-tagged S104A mutant PED/PEA-15 (B), and His-tagged S116A mutant PED/PEA-15 (C). Absence of signal in PED/PEA-15 S116A mutant demonstrates antibody specificity.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lysates of PMA-stimulated Cos7 cells transfected with HA-tagged wild-type PED/PEA-15 (A), His-tagged S104A mutant PED/PEA-15 (B), and His-tagged S116A mutant PED/PEA-15 (C). Absence of signal in PED/PEA-15 S116A mutant demonstrates antibody specificity.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (20 µg lysate) of L6 (lane 1) and L6 treated for 10 minutes with 200 ng/mL of Insulin (lane 2). The blots were probed with Anti-PEA-15 (pS116) Rabbit Polyclonal Antibody (Product # 44-836G, 1:500-1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Recombinant Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 15 kDa band corresponding to PEA-15 (pS116) was observed across the treated cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).