MA5-29133

antibody from Invitrogen Antibodies

Targeting: CD84 hCD84, mCD84, SLAMF5

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29133

Provider

Invitrogen Antibodies

Product name

CD84 Recombinant Rabbit Monoclonal Antibody (6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

6

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD84 in Lane A: RAW264.7 Whole Cell Lysate (30 µg). Samples were probed using a CD84 Monoclonal Antibody (Product # MA5-29133) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:39 kDa. Observed band size:39 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD84 Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29133) at 1:500 dilution. Lane A: RAW264.7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 39 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD84 Immunoprecipitation using: Lane A: 0.5 mg Raw264.7 Whole Cell Lysate 0.5 µL with CD84 Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29133) and 15 µL of 50 % Protein G agarose. Primary antibody: CD84 Recombinant Rabbit Monoclonal Antibody (6), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa.