Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 101-M54 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- TIE-2
- Antibody type
- Monoclonal
- Antigen
- recombinant human soluble TIE-2
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
AMDLILINSLPLVSDAETSLTCIASGWRPHEPITI
GRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREK
ASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPA
TLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFI
HSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGN
LFTSAFTRLIVRRCEAQKWGPECNHLCTACMNNGV
CHEDTGECICPPGFMGRTCEKACELHTFGRTCKER
CSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEAC
HPGFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQG
LQCEREGIQRMTPKIVDLPDHIEVNSGKFNPICKA
SGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVA
IFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVK
VLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIK
SKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTE
YELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPR
GLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRS
VQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARV
NTKAQGEWSEDLTAWTLSDILPPQPENIKISNITH
SSAVISWTILDGYSISSITIRYKVQGKNEDQHVDV
KIKNATITQYQLKGLEPETAYQVDIFAENNIGSSN
PAFSHELVTRSDKTHTCPPCPAPELLGGPSVFLFP
PKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYV
DGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWL
NGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVY
TLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESN
GQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQ
GNVFSCSVMHEALHNHYTQKSLSLSPGK- Isotype
- IgG
- Antibody clone number
- (#tek16)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood.
Soluble Tie2 and Flt1 extracellular domains in serum of patients with renal cancer and response to antiangiogenic therapy.
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Reusch P, Barleon B, Weindel K, Martiny-Baron G, Gödde A, Siemeister G, Marmé D
Angiogenesis 2001;4(2):123-31
Angiogenesis 2001;4(2):123-31
Soluble Tie2 and Flt1 extracellular domains in serum of patients with renal cancer and response to antiangiogenic therapy.
Harris AL, Reusch P, Barleon B, Hang C, Dobbs N, Marme D
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Jul;7(7):1992-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Jul;7(7):1992-7
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Norgall S, Papoutsi M, Rössler J, Schweigerer L, Wilting J, Weich H
BMC Cancer ;7(1):105
BMC Cancer ;7(1):105
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Quantification of soluble and cellular TIE-2 by sandwich ELISA. Stimulation of sTIE-2 release by PMA and basic FGF in comparison to untreated cells. For capturing anti-human TIE-2 Cl.16 [#101-M54] was used, for the detection a mixture of biotinylated anti-human TIE-2 Cl. 2 [#101-M50] and Cl.9 [#101-M52].
- Sample type
- HUVEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Effects of PMA treatment on TIE-2 . HUVECs (passage 1) were stimulated for the indicated periods of time with PMA at 25 ng/ml or left untreated. Western blot analysis for the presence of TIE-2 protein by immunoprecipitation using antibodies directed against the extracellular domain of human TIE-2 (IP: TEK16 [#101-M54]; Western: TEK2 [#101-M50]).
- Sample type
- HUVEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- IHC with cryo sections of human spleen.
- Sample type
- Human Spleen
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-mouse IgG-PE was used.
- Sample type
- HDLEC