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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Immunohistochemistry [1]
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- Product number
- A01274-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TIE2/TEK Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for TIE2/TEK detection. Tested with WB, IHC-P in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Saikosaponin-d regulates angiogenesis in idiopathic pulmonary fibrosis through angiopoietin/Tie-2 pathway.
Transplantation of Human Umbilical Cord Blood Mononuclear Cells Attenuated Ischemic Injury in MCAO Rats via Inhibition of NF-κB and NLRP3 Inflammasome.
Exercise improves recovery after ischemic brain injury by inducing the expression of angiopoietin-1 and Tie-2 in rats.
Eukaryotic expression of extracellular ligand binding domains of murine Tie-2 and its anti-angiogenesis effect in SGC-7901 cell lines.
Rapamycin and cyclosporine have different effects on expression of Ang-1 and Ang-2 and Tie2 in rat renal allograft with chronic allograft nephropathy.
Expressions of Angiopoietin-1, Angiopoietin-2, and Tie2 and their roles in rat renal allografts with chronic allograft nephropathy.
Wu Y, Zhang J, Wang X, Xu Y, Zheng J
Acta histochemica 2023 Dec;125(8):152100
Acta histochemica 2023 Dec;125(8):152100
Transplantation of Human Umbilical Cord Blood Mononuclear Cells Attenuated Ischemic Injury in MCAO Rats via Inhibition of NF-κB and NLRP3 Inflammasome.
Liu L, Cen J, Man Y, Li J, Zhang D, Wang F, Li J, Ma J, Wang X, Ji B
Neuroscience 2018 Jan 15;369:314-324
Neuroscience 2018 Jan 15;369:314-324
Exercise improves recovery after ischemic brain injury by inducing the expression of angiopoietin-1 and Tie-2 in rats.
Zheng Q, Zhu D, Bai Y, Wu Y, Jia J, Hu Y
The Tohoku journal of experimental medicine 2011 Jul;224(3):221-8
The Tohoku journal of experimental medicine 2011 Jul;224(3):221-8
Eukaryotic expression of extracellular ligand binding domains of murine Tie-2 and its anti-angiogenesis effect in SGC-7901 cell lines.
Yang H, Yang K, Hu JK, Tang H, Zhang B, Chen ZX, Wang YJ, Chen JP
Journal of gastroenterology and hepatology 2010 Feb;25(2):345-51
Journal of gastroenterology and hepatology 2010 Feb;25(2):345-51
Rapamycin and cyclosporine have different effects on expression of Ang-1 and Ang-2 and Tie2 in rat renal allograft with chronic allograft nephropathy.
Ma X, Lu YP, Yang L, Song J, Luo GH, Shi YJ, Li YP
Transplantation proceedings 2008 Oct;40(8):2804-7
Transplantation proceedings 2008 Oct;40(8):2804-7
Expressions of Angiopoietin-1, Angiopoietin-2, and Tie2 and their roles in rat renal allografts with chronic allograft nephropathy.
Ma X, Lu YP, Yang L, Song J, Luo GH, Shi YJ, Li YP
Transplantation proceedings 2008 Oct;40(8):2795-9
Transplantation proceedings 2008 Oct;40(8):2795-9
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of TEK using anti-TEK antibody (A01274-1).TEK was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
