PA007389.h1Fs

antibody from Syd Labs, Inc.

Targeting: FCGR3A CD16, CD16a, FCG3, FCGR3

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

PA007389.h1Fs

Provider

Syd Labs, Inc.

Product name

Anti-mouse CD16/CD32 Antibody

Antibody type

Monoclonal

Description

The recombinant 2.4G2 antibody binds to mouse CD16 (Fc¦ÃIII) and CD32 (Fc¦ÃII) receptor proteins. The major component of some commercial Fc block reagents is the 2.4G2 antibody. The rat, mouse, and human IgG Fc binds to mouse CD64 (Fc¦ÃI). Thus, the recombinant 2.4G2 antibodies can be used to block non-specific binding of IgGs to mouse Fc receptors in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. Customers may choose different IgG isotypes for their own applications. The most popular mouse Fc blocking reagents include: Anti-Mouse CD16/CD32 (Clone 2.4G2): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for Fc¦ÃRII (CD32) and Fc¦ÃRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse Fc¦ÃRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity. Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: for general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. Purified rat IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls. Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking. Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. Best choice of mouse Fc blocking reagents based on your application are: For flow cytometry: anti-mouse CD16/CD32 (Clone 2.4G2). For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. For Western Blot and ELISA: BSA or FBS. For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

Reactivity

Mouse

Host

Human

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

2.4G2

Vial size

1 mg, 5 mg, 25 mg

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70¡ãC as supplied. 1 month from date of receipt, 2 to 8¡ãC as supplied.