Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-1018-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-ATF6 Antibody
- Antibody type
- Monoclonal
- Description
- ATF6, also named as Cyclic AMP-dependent transcription factor ATF-6 alpha is a member of bZIP family, ATF subfamily. ATF6 forms homodimer and heterodimer with ATF6-beta. ATF6 acts by activating specific unfolded protein response target genes during endoplasmic reticulum stress. ATF6 is a ubiquitously expressed transcription factor located at endoplasmic reticulum (ER) membrane. During ER stress an approximately 50 kDa fragment containing the N-terminal cytoplasmic domain is cleaved by proteolysis and is translocated into the nucleus. N-glycosylation may serve as a sensor for ER homeostasis. For the binding of ATF6 to ERSE, ESRE should first bind to another transcription factor called Nuclear Transcription Factor Y (NF-Y). ATF6 could also be involved in activation of transcription by interacting with serum response factor. ATF6 is a 74 kDa protein and is highly expressed in kidney, liver, colon and mammary gland.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant ATF6 p
rotein (amino acids 1-400) was used
as the immunogen for this antibody
.- Isotype
- IgG
- Antibody clone number
- ABM1A40
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 µg/ml on K562 lysate.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 µg/ml on (1) Blank plasmid transfected HEK293 cells, (2) Partial length ATF6 plasmid transfected HEK293 cells and (3) Fulll length ATF6 plasmid transfected HEK293 cells.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular staining of full length ATF6 plasmid and blank plasmid transfected HEK293 cells. 0.5 ug of anti-ATF6 antibody was used. Green represents blank plasmid, Red represents ATF6 expression plasmid. Goat anti-mouse PE was used as secondary antibody.
- Protocol
- Protocol