Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-85935 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effect of Propionic Acid on Diabetes-Induced Impairment of Unfolded Protein Response Signaling and Astrocyte/Microglia Crosstalk in Rat Ventromedial Nucleus of the Hypothalamus.
Natrus LV, Osadchuk YS, Lisakovska OO, Labudzinskyi DO, Klys YG, Chaikovsky YB
Neural plasticity 2022;2022:6404964
Neural plasticity 2022;2022:6404964
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in Hela cell lysate using a ATF6 Polyclonal antibody (Product # PA5-85935) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 in Hela cell lysate using a ATF6 Polyclonal antibody (Product # PA5-85935) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of ATF6 in HUVEC cells using a ATF6 Polyclonal antibody (Product # PA5-85935) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of ATF6 in A431 cells using a ATF6 Polyclonal antibody (Product # PA5-85935) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATF6 of paraffin-embedded rat brain tissue using a ATF6 Polyclonal antibody (Product #PA5-85935). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATF6 of paraffin-embedded Human kidney tissue using a ATF6 Polyclonal antibody (Product #PA5-85935). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of ATF6 in K562 cells using a ATF6 Polyclonal Antibody (Product # PA5-85935) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Effects of administration of metformin and PA on the major components of unfolded protein response system--PERK, ATF6, and IRE1. Immunoblotting analysis of PERK, ATF6, and IRE1 in rat VMH: the bar graphs of (a) PERK, (c) ATF6, and (e) IRE1 protein content are presented; (f) representative immunoblots are shown, and data are quantified using tubulin as a loading control for hypothalamus lysates. Quantitative RT-PCR of (b) Perk and (d) Atf6 in rat VMH: data were normalized to beta -actin and pooled from three independent experiments ( n = 6 rats/group). All data are shown as means +- SEM; * p < 0.05 vs. control, ** p < 0.05 vs. T2DM, # p < 0.05 vs. metformin administration, and @ p < 0.05 vs. PA administration.