PA5-22332

antibody from Invitrogen Antibodies

Targeting: RGS4 SCZD9

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22332

Provider

Invitrogen Antibodies

Product name

RGS4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Raji, mouse cerebellum, rat brain. Predicted reactivity: Mouse (95%), Rat (96%), Pig (95%), Rabbit (95%), Sheep (96%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.21 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Regulator of G-Protein Signaling 4 (RGS4) Controls Morphine Reward by Glutamate Receptor Activation in the Nucleus Accumbens of Mouse Brain.

Kim J, Lee S, Kang S, Jeon TI, Kang MJ, Lee TH, Kim YS, Kim KS, Im HI, Moon C
Molecules and cells 2018 May 31;41(5):454-464

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 RGS4 controlled morphine-induced reward in the NAc (A) Schematic representation of the shRNA-targeting RGS4 construct. (B) Injection target site in the mouse brain (NAc; red circle). (C) Representative photographs of eGFP expression in the NAc of mice injected with Lv-eGFP and Lv-shRNA-eGFP. (D) Representative immunoblots of RGS4 expression in the NAc of mice injected with Lv-eGFP and Lv-shRNA-eGFP. All data are expressed as means +- SEM; n = 6 for each group. (E) Schematic diagram of the schedule for the morphine CPP test. (F) Mice infected with Lv-shRNA-eGFP in the NAc showed an increased response to morphine (15 mg/kg, i.p.) in the CPP paradigm. All data are expressed as means +- SEM (n = 6 for each group). Data were analyzed with unpaired t -tests. * p < 0.05 vs. the Lv-eGFP infected group.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Morphine induced the activation of ionotropic glutamate receptors in primary NAc/striatal neurons To examine the effects of morphine on glutamate receptors in NAc/striatal neurons, the neurons (9 DIV) were incubated with morphine (10 muM) for 3 days. (A) Representative photographs of immunoblots for RGS4, phospho-GluR1 (Ser831), total-GluR1, phospho-NR2A (Tyr1325), total-NR2A, phospho-CaMKII (Thr286), total-CaMKII, phospho-PKA-C (Thr197), and total-PKA-C. (B-F) Bar graphs showing semi-quantitative analyses of the phosphorylation levels of GluR1, NR2A, CaMKII, and PKA-C in NAc/striatal neurons after vehicle and morphine treatment. All data are expressed as means +- SEM; n = 6 for each group and were analyzed with unpaired t-tests. p < 0.05 vs . vehicle-treated control and ** p < 0.01 vs. vehicle-treated control.