Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003795-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003795-M01, RRID:AB_540005
- Product name
- Mouse Monoclonal Ketohexokinase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KHK - ketohexokinase (fructokinase)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - KHK expression in human spleen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK over-expressed 293 cell line, cotransfected with KHK Validated Chimera RNAi ( Cat # H00003795-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KHK monoclonal antibody (M01), clone 1H6-2B6 (Cat # H00003795-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.Lane 1: KHK transfected lysate(32.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - KHK monoclonal antibody (M01), clone 1H6-2B6. Analysis of KHK expression in human liver.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Detection limit for recombinant GST tagged KHK is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of monoclonal antibody to KHK on formalin-fixed paraffin-embedded human spleen tissue. Antibody concentration 1 ~ 10 ug/ml.