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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-112588 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KHK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.51 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KHK in multiple sample types using a KHK polyclonal antibody (Product # PA5-112588) at a concentration of 3.6 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: Mouse liver tissue, Lane 2: Mouse kidney tissue, Lane 3: Rat kidney tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of KHK in Mouse liver tissue using a Polyclonal antibody (Product #PA5-112588). Samples were treated with 8 µg of the primary antibody and an HRP-conjugated Protein G secondary antibody was used at a dilution of 1:2,000. Lane 1: Rabbit control IgG, Lane 2: 500 µg of Mouse liver tissue, Lane 3: 10 µg of Mouse liver tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded KHK in human liver tissue using a KHK Polyclonal antibody (Product # PA5-112588) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded KHK in human colon cancer using a KHK Polyclonal antibody (Product # PA5-112588) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded KHK in human colon cancer using a KHK Polyclonal antibody (Product # PA5-112588) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of KHK in Mouse liver tissue using a Polyclonal antibody (Product #PA5-112588). Samples were treated with 8 µg of the primary antibody and an HRP-conjugated Protein G secondary antibody was used at a dilution of 1:2,000. Lane 1: Rabbit control IgG, Lane 2: 500 µg of Mouse liver tissue, Lane 3: 10 µg of Mouse liver tissue.
