PA5-96292

antibody from Invitrogen Antibodies

Targeting: KIFAP3 FLA3, KAP-1, KAP3, SMAP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96292

Provider

Invitrogen Antibodies

Product name

KAP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MQGEDARYLK RKVKGGNIDV HPSEKALIVH YEVEATILGE MGDPMLGERK ECQKIIRLKS LNANTDITSL ARKVVEECKL IHPSKLNEVE QLLYYLQNRR DSLSGKEKKE KSSKPKDPPP FEGMEIDEVA NINDMDEYIE LLYEDIPDKV RGSALILQLA RNPDNLEELL LNETALGALA RVLREDWKQS VELATNIIYI; Positive Samples: LO2, SH-SY5Y, Mouse brain, Mouse testis, Mouse lung, Rat lung

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.19 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KAP3 in extracts of various cell lines. Samples were incubated with KAP3 Polyclonal antibody (Product # PA5-96292) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of KAP3 in L929 cells. Samples were incubated with KAP3 Polyclonal antibody (Product # PA5-96292) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of KAP3 in H9C2 cells. Samples were incubated with KAP3 Polyclonal antibody (Product # PA5-96292) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of KAP3 in U2OS cells. Samples were incubated with KAP3 Polyclonal antibody (Product # PA5-96292) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of KAP3 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg KAP3 Polyclonal antibody (Product # PA5-96292). Western blot was performed from the immunoprecipitate using KAP3 Polyclonal antibody (Product # PA5-96292) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of KAP3 was performed in 200 µg extracts of HeLa cells using KAP3 Polyclonal Antibody (Product # PA5-96292). Western blot was performed from the immunoprecipitate using KAP3 Polyclonal Antibody.