12700-1-AP

antibody from Invitrogen Antibodies

Targeting: KIFAP3 FLA3, KAP-1, KAP3, SMAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12700-1-AP

Provider

Invitrogen Antibodies

Product name

KIFAP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SQHDGPTKN LFIDYVGDLA AQISNDEEEE FVIECLGTLA NLTIPDLDWE LVLKEYKLVP YLKDKLKPGA AEDDLVLEVV IMIGTVSMDD SCAALLAKSG IIPALIELLN AQQEDDEFVC QIIYVFYQMV FHQATRDVII KETQAPAYLI DLMHDKNNEI RKVCDNTLDI IAEYDEEWAK KIQSEKFRWH NSQWLEMVES RQMDESEQYL YGDDRIEPYI HEGDILERPD LFYNSDGLIA SEGAISPDFF NDYHLQNGDV VGQHSFPGSL GMDGFGQPVG ILGRPATAYG FRPDEPYYYG YGS (491-792 aa encoded by BC028679)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-KIFAP3 (IP:12700-1-AP, 4ug; Detection:12700-1-AP 1:500) with mouse testis tissue lysate 4000ug.