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- Antibody Data
- Antigen structure
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- Product number
- MAB370-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ALK-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human ALK-1 in direct ELISAs and Western blots. Shows less than 1% cross-reactivity with recombinant mouse ALK-1, recombinant human (rh) Activin RIA, or rhActivin RIB.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human ALK-1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ALK-1 at approximately 63 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human ALK-1 Monoclonal Antibody (Catalog # MAB370). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human ALK-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALK-1 Monoclonal Antibody (Catalog # MAB370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALK-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
