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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [2]
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- Product number
- MAB370-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ALK-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human ALK-1 in direct ELISAs and Western blots. Shows less than 1% cross-reactivity with recombinant mouse ALK-1, recombinant human (rh) Activin RIA, or rhActivin RIB.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P37023- Isotype
- IgG
- Antibody clone number
- 117720
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Therapeutic action of tranexamic acid in hereditary haemorrhagic telangiectasia (HHT): regulation of ALK-1/endoglin pathway in endothelial cells.
Antitumor activity of ALK1 in pancreatic carcinoma cells.
Fernandez-L A, Garrido-Martin EM, Sanz-Rodriguez F, Ramirez JR, Morales-Angulo C, Zarrabeitia R, Perez-Molino A, Bernabéu C, Botella LM
Thrombosis and haemostasis 2007 Feb;97(2):254-62
Thrombosis and haemostasis 2007 Feb;97(2):254-62
Antitumor activity of ALK1 in pancreatic carcinoma cells.
Ungefroren H, Schniewind B, Groth S, Chen WB, Müerköster SS, Kalthoff H, Fändrich F
International journal of cancer 2007 Apr 15;120(8):1641-51
International journal of cancer 2007 Apr 15;120(8):1641-51
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human ALK-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALK-1 Monoclonal Antibody (Catalog # MAB370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALK-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human ALK-1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ALK-1 at approximately 63 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human ALK-1 Monoclonal Antibody (Catalog # MAB370). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
