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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F50788 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ALK1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 38-68 from the human protein was used as the immunogen for this ALK1 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the ALK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of ALK1 antibody and mouse heart tissue lysate. Predicted molecular weight ~60 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of ALK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (2).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ALK1 (2, 4); ALK1 antibody used at 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), and 5% BSA in TBST (3 and 4). Data courtesy of Kenneth Finnson, Montreal General Hospital.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the ALK1 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using ALK1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
