Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3475 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3475, RRID:AB_1570902
- Product name
- ACVRL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ACVRL1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references LC-MS/MS analysis of apical and basolateral plasma membranes of rat renal collecting duct cells.
Yu MJ, Pisitkun T, Wang G, Shen RF, Knepper MA
Molecular & cellular proteomics : MCP 2006 Nov;5(11):2131-45
Molecular & cellular proteomics : MCP 2006 Nov;5(11):2131-45
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ACVRL1 (arrow) using rabbit ACVRL1 polyclonal antibody (Cat # PAB3475). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ACVRL1 polyclonal antibody (Cat # PAB3475) in human chondrocytes (C-28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1 (lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1 : 1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ACVRL1 polyclonal antibody (Cat # PAB3475) in mouse heart tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ACVRL1 polyclonal antibody (Cat # PAB3475) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using ACVRL1 polyclonal antibody (Cat # PAB3475)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
