PA5-95285

antibody from Invitrogen Antibodies

Targeting: FMO1

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95285

Provider

Invitrogen Antibodies

Product name

FMO1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human hepatocellular carcinoma tumor tissue, human HepG2 whole cell, human HUH7 whole cell, rat liver tissue, mouse liver tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.

Huang S, Howington MB, Dobry CJ, Evans CR, Leiser SF
Frontiers in cell and developmental biology 2021;9:630188

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 1 C. elegans FMO-2 and mammalian FMOs improve stress resistance to oxidative stress. (A) Amino acid sequence alignment of FMOs across species with the regions containing the eight essential residues denoted as red arrowheads in the catalytic active site among reconstructed ancestral mammalian FMO5, C. elegans FMO-2, and mouse FMO1-5. (B) Wild-Type and FMO-2 overexpressing (FMO-2 OE) worm survival curves on paraquat stress. Each strain was placed on NGM (Nematode Growth Medium) containing 5 mM paraquat from the fourth larvae stage (L4) at Day 0. Survival was quantified every day until all worms were dead. The difference between the survival curves is denoted with the log-rank test p -value. (C) FMO1-5 protein levels in FMO1-5 OE and control cells of both HEK293A and HepG2. (D) FMO5 OE and control cell survival curves on paraquat stress in HEK293A and HepG2. HEK293A cells or HepG2 cells stably expressing FMO5 or empty vector pDEST were subjected to indicated increasing doses of paraquat. (E) LD 50 values of FMO1-5 OE cells compared to the control cells on paraquat stress in HEK293A and HepG2. HEK293A cells or HepG2 cells stably expressing FMO1-5 or empty vector pDEST were subjected to indicated increasing doses of paraquat. (F) FMO5 OE and control cell survival curves on cadmium stress in HEK 293A and HepG2. HEK293A cells or HepG2 cells stably expressing FMO5 or empty vector pDEST were subjected to indicated increasing doses of cadmium. (G) LD 50 values of FMO-OE cells c