LS-C782873

antibody from LSBio

Targeting: DARS2 FLJ10514, mtAspRS

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C782873

Provider

LSBio

Product name

DARS2 Antibody LS-C782873

Antibody type

Polyclonal

Description

Antigen Affinity purification

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot testing of human 1) A431, 2) K562, 3) A549, 4) PC-3, 5) U-2 OS, 6) Caco-2 and 7) HEK293 lysate with DARS2 antibody at 0.5ug/ml. Predicted molecular weight ~74 kDa.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human intestinal cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human intestinal cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human breast cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human breast cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC staining of FFPE human lung cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC staining of FFPE human intestinal cancer with DARS2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.