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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- R30852 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SIP Antibody / SIAH Interacting Protein / CACYBP
- Antibody type
- Polyclonal
- Description
- This highly specific SIP antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SIP antibody and Lane 1: rat liver ; 2: (r) brain; 3: (r) spleen; 4: human SMMC-7721; 5: (h) COLO320; 6: (h) SW620; 7: (h) 293T cell lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human MCF7, 2) human RT4, 3) human SW620, 4) human U-251, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with SIP antibody. Predicted molecular weight ~27 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC: A549 cells
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with SIP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver cancer tissue with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat brain with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HL60 cells with SIP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIP antibody.
