LS-C672262

antibody from LSBio

Targeting: CACYBP S100A6BP, SIP

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C672262

Provider

LSBio

Product name

CACYBP Antibody LS-C672262

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate. All lanes: CACYBP antibody at 2.7 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27, 21, 9 KDa. Observed band size: 27 KDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Positive Western Blot detected in 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate. All lanes: CACYBP antibody at 2.7 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27, 21, 9 KDa. Observed band size: 27 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating CACYBP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CACYBP Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CACYBP Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CACYBP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CACYBP Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CACYBP Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CACYBP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CACYBP Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CACYBP Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)