PA5-117823

antibody from Invitrogen Antibodies

Targeting: CACYBP S100A6BP, SIP

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117823

Provider

Invitrogen Antibodies

Product name

Calcyclin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Calcyclin in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Calcyclin Polyclonal Antibody (Product # PA5-117823, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Calcyclin in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Calcyclin Polyclonal Antibody (Product # PA5-117823, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Calcyclin in A431 cells. Samples were treated with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-117823) with a dilution of 1:200 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcyclin Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with Calcyclin Polyclonal Antibody (Product # PA5-117823) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Calcyclin Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 30 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Calcyclin in Lane A: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117823) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 26 kDa , Observed band size : 30 kDa .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of Calcyclin in human large intestine. Samples were incubated with polyclonal antibody (Product # PA5-117823) with a dilution of 1:500 .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Calcyclin in human large intestine with Calcyclin Polyclonal Antibody (Product # PA5-117823, 1:500 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of Calcyclin in human urinary bladder. Samples were incubated with polyclonal antibody (Product # PA5-117823) with a dilution of 1:500 .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Calcyclin Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with Calcyclin Polyclonal Antibody (Product # PA5-117823) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Calcyclin Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 30 kDa.