19662-1-AP

antibody from Proteintech Group

Targeting: HK1

Provider product page for 19662-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [71]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 19662-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#19662-1-AP, RRID:AB_10859778
Product name: Hexokinase 1 antibody
Antibody type: Polyclonal
Description: KD/KO validated Hexokinase 1 antibody (Cat. #19662-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HK1 protein structure - 19662-1-AP shown in red.

Submitted references TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis.

Barone C, Wang R, Cooke S, Ng HP, Ferreira RS, Miranda HC, Qi X
Acta neuropathologica 2026 Mar 16;151(1)

Thallium exposure disturbs gut microbiota composition, metabolic function, and host gut energy metabolism in aged mice.

Yao H, Su Q, Dai Q, Li L, Li D
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2026 Jan;207:115793

Type II Alveolar Epithelial Cells Promote Sepsis-Induced Immunosuppression in Alveolar Macrophages via Exosomal lncRNA Rmrp Release.

Liu C, Xuan W, Cao S, Jia H, Wu Q, Tan X, Wang Q, Li X, Ding L, Xiong Y, Zhao M, Zheng L, Xi Y, Tan J, Li R, Zhang X, Liu W, Wu X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(1):e00376

Developmental lead exposure induces neurotoxicity via glycolytic failure, glial activation, and miR-144 downregulation in male rat offspring.

Zeid SMA, Mahmoud MO, Boshra SA, Gomaa SB
Tissue & cell 2026 Aug;101:103462

Ferulic acid inhibits Tau-441 liquid-liquid phase separation to restore glycolytic metabolism.

Lin WJ, Li CY, Shi WP, Liu TA, Wang YJ, Li YJ
International journal of biological macromolecules 2026 Apr;358:151806

Hexokinase-2 as a Therapeutic Target: Alleviating Herpes Simplex Keratitis Through Metabolic Reprogramming.

Jiang D, Sun Y, Yu X, Wang R, Zhang Y, Yu J, Xie S, Cai Y, Luo Y, Chen W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(34):e03690

PKM2 alleviates mitochondrial oxidative stress and neuronal apoptosis through metabolic and non-metabolic pathways to protect SOD1(G93A) mice.

Zhang Z, Guo X, Liu Y, Ke P, Meng Y, Gu J, Hu L, Yuan Z, Duan R, Luo J, Xiao F
Free radical biology & medicine 2025 Oct;238:1-16

The Role of PI3K/AKT/HIF-1α Pathway in the Effect of Nano-TiO(2) on Lactate Production in TM4 Cells.

Chang H, Zhao S, Lei Y, Hu Y, Yan Y, Song G
Journal of applied toxicology : JAT 2025 Mar;45(3):492-502

De-SUMO1ylated hexokinase 1 selectively degraded by ubiquitin-proteasome pathway promotes boar sperm capacitation in vitro.

Yao S, Xu Q, Lv Y, Zhang Y, Li H, Zhang J, Yao Z, An Y, Chen X, Jin Y
Animal reproduction science 2025 Mar;274:107794

GRASP55 regulates sorting and maturation of the lysosomal enzyme β-hexosaminidase A.

Akaaboune SR, Javed A, Bui S, Wierenga A, Wang Y
Molecular biology of the cell 2025 Mar 1;36(3):ar30

Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model.

Ding X, Chen C, Zhao H, Dai B, Ye L, Song T, Huang S, Wang J, You T
Neural regeneration research 2025 Mar 1;20(3):858-872

Dynamic profiles of early biological responses to predict the treatment efficacy of proton therapy in liver cancer assessed with in vivo kinetic [18F]-FDG PET/MRI.

Chung YH, Cho IC, Jhang F, Weng CC, Lin G, Yu CF, Chen FH
Radiation oncology (London, England) 2025 Jun 6;20(1):96

Chondrocyte-targeted α-Solanine through HIF-1α regulating glycolysis to reduce the ferroptosis of chondrocyte in osteoarthritis.

Li W, Liu Y, Wei M, Yang Z, Tang H, Huang W
International immunopharmacology 2025 Jun 26;159:114841

Gastrodin alleviates Aβ(25-35)-induced glycolytic dysfunction via activating PI3K/AKT/BACH1 signaling in Alzheimer's disease models.

Liu TT, Yang X, Lei HP, Hu YT, Wu LN, Wei AH, Ji XH, Liu J, Jin H, Shi JS, Zhou SY, Jin F
Experimental neurology 2025 Jul;389:115225

Functionalized Biomimetic Nanoparticles Targeting the IL-10/IL-10Rα/Glycolytic Axis in Synovial Macrophages Alleviate Cartilage Degeneration in Osteoarthritis.

Li W, Liu Y, Wei M, Yang Z, Li Z, Guo Z, Yan L, Lu Y, Tang H, Li B, Huang W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(27):e2504768

Cardiomyocyte OTUD1 drives diabetic cardiomyopathy via directly deubiquitinating AMPKα2 and inducing mitochondrial dysfunction.

Han X, Zheng R, Zhang J, Liu Y, Li Z, Liu G, Zheng J, Li W, Liang Z, Wang M, Yu J, Shi Q, Ying H, Liang G
Nature communications 2025 Jul 19;16(1):6668

Self-organizing glycolytic waves tune cellular metabolic states and fuel cancer progression.

Zhan H, Pal DS, Borleis J, Deng Y, Long Y, Janetopoulos C, Huang CH, Devreotes PN
Nature communications 2025 Jul 1;16(1):5563

A mitochondria-to-nucleus regulation mediated by the nuclear-translocated mitochondrial lncRNAs.

Li J, Bai R, Zhou Y, Song X, Li L
PLoS genetics 2025 Jan;21(1):e1011580

Daurisoline inhibits glycolysis of lung cancer by targeting the AKT-HK2 axis.

Tan SM, Luo L, He YF, Li W, Wan XX
Cancer biology & therapy 2025 Dec;26(1):2442556

Mitochondrial membrane junction-mediated ATP channeling drives activity-dependent glucose metabolism.

Yang D, Molinaro G, Nijem N, Zhang C, Penny D, Bai M, Lin MJ, Wen X, Pena S, Wansapura J, Gaitanou M, Matsas R, Wang B, Baniasadi H, Han Y, Gibson J, Huber K, Zeng X
bioRxiv : the preprint server for biology 2025 Dec 19;

Heme allocation in eukaryotic cells relies on mitochondrial heme export through FLVCR1b to cytosolic GAPDH.

Jayaram DT, Sivaram P, Biswas P, Dai Y, Sweeny EA, Stuehr DJ
Nature communications 2025 Aug 26;16(1):7972

H4K79 and H4K91 histone lactylation, newly identified lactylation sites enriched in breast cancer.

Liu J, Zhao L, Yan M, Jin S, Shang L, Wang J, Wang Q, Zhao S, Shen Z, Liu T, Wu H, Pang D
Journal of experimental & clinical cancer research : CR 2025 Aug 23;44(1):252

Jiang Y, Wang Z, Zhang K, Hu Y, Shang D, Jiang L, Huang M, Wang B, He X, Wu Z, Yan X, Zhang X
Journal of clinical periodontology 2025 Apr;52(4):622-636

The long noncoding RNA MIR4435-2HG enhances the migration, promotion, and glycolysis of nonsmall cell lung cancer cells by targeting the miR-371a-5p/SOX2/PI3K/Akt axis.

Yang J, Su Y, Wang Y, Gao K, Li C, Li M
SAGE open medicine 2024;12:20503121241289290

Study on the mechanisms of defective spermatogenesis induced by TiO(2) NPs based on 3D blood-testis barrier microfluidic chip.

Dong R, Li L, Chang H, Song G, Liu S
Toxicology 2024 Sep;507:153888

Inhibition of KIAA1429/HK1 axis enhances the sensitivity of liver cancer cells to sorafenib by regulating the Warburg effect.

Liu D, Shan M, Zeng R, He M, Dai X, Lu L, Yang M, He H, Zhang Y, Xiang L, Chen A, Sun L, He F, Lian J
Biochemical pharmacology 2024 Sep;227:116419

Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation.

Zhou J, Zhang L, Peng J, Zhang X, Zhang F, Wu Y, Huang A, Du F, Liao Y, He Y, Xie Y, Gu L, Kuang C, Ou W, Xie M, Tu T, Pang J, Zhang D, Guo K, Feng Y, Yin S, Cao Y, Li T, Jiang Y
Cell metabolism 2024 Sep 3;36(9):2054-2068.e14

GRASP55 Regulates Sorting and Maturation of the Lysosomal Enzyme β-Hexosaminidase A.

Akaaboune SR, Javed A, Bui S, Wierenga A, Wang Y
bioRxiv : the preprint server for biology 2024 Oct 17;

Identification of TPI1 As a potential therapeutic target in pancreatic cancer with dependency of TP53 mutation using multi-omics analysis.

Toyoda T, Miura N, Kato S, Masuda T, Ohashi R, Matsushita A, Matsuda F, Ohtsuki S, Katakura A, Honda K
Cancer science 2024 Nov;115(11):3622-3635

BAP1 inactivation promotes lactate production by leveraging the subcellular localization of LDHA in melanoma.

Zheng G, Shi J, Li Q, Jin X, Fang Y, Zhang Z, Cao Q, Zhu L, Shen J
Cell death discovery 2024 Nov 26;10(1):483

Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma.

Yao X, Kong L, Qiao Y, Brand D, Li J, Yan Z, Zheng SG, Qian Y, Fan C
Cell reports. Medicine 2024 Nov 19;5(11):101791

Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway.

Liu J, Wei AH, Liu TT, Ji XH, Zhang Y, Yan F, Chen MX, Hu JB, Zhou SY, Shi JS, Jin H, Jin F
The FEBS journal 2024 May;291(10):2221-2241

Generation of human alveolar epithelial type I cells from pluripotent stem cells.

Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, Kotton DN
Cell stem cell 2024 May 2;31(5):657-675.e8

The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis.

Zhao L, Yu N, Zhai Y, Yang Y, Wang Y, Yang Y, Gong Z, Zhang Y, Zhang X, Guo W
Cell death & disease 2024 Jul 13;15(7):502

Dual impacts of serine/glycine-free diet in enhancing antitumor immunity and promoting evasion via PD-L1 lactylation.

Tong H, Jiang Z, Song L, Tan K, Yin X, He C, Huang J, Li X, Jing X, Yun H, Li G, Zhao Y, Kang Q, Wei Y, Li R, Long Z, Yin J, Luo Q, Liang X, Wan Y, Zheng A, Lin N, Zhang T, Xu J, Yang X, Jiang Y, Li Y, Xiang Y, Zhang Y, Feng L, Lei Z, Shi H, Ma X
Cell metabolism 2024 Dec 3;36(12):2493-2510.e9

Nanoparticles for inducing Gaucher disease-like damage in cancer cells.

Yue C, Lu W, Fan S, Huang Z, Yang J, Dong H, Zhang X, Shang Y, Lai W, Li D, Dong T, Yuan A, Wu J, Kang L, Hu Y
Nature nanotechnology 2024 Aug;19(8):1203-1215

N(6)-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing.

Wang A, Zeng Y, Zhang W, Zhao J, Gao L, Li J, Zhu J, Liu Z, Huang JA
Cellular & molecular biology letters 2024 Aug 2;29(1):106

Yu Linzhu alleviates primary ovarian insufficiency in a rat model by improving proliferation and energy metabolism of granulosa cells through hif1α/cx43 pathway.

Ruan X, Wang P, Wei M, Yang Q, Dong X
Journal of ovarian research 2024 Apr 26;17(1):89

Magnetic Fields Reduce Apoptosis by Suppressing Phase Separation of Tau-441.

Lin WJ, Shi WP, Ge WY, Chen LL, Guo WH, Shang P, Yin DC
Research (Washington, D.C.) 2023;6:0146

Suppression of hnRNP A1 binding to HK1 RNA leads to glycolytic dysfunction in Alzheimer's disease models.

Ji XH, Liu TT, Wei AH, Lei HP, Chen Y, Wu LN, Liu J, Zhang Y, Yan F, Chen MX, Jin H, Shi JS, Zhou SY, Jin F
Frontiers in aging neuroscience 2023;15:1218267

Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction.

Koleini N, Meddeb M, Keykhaei M, Kwon S, Zhao L, Hahn V, Sharma K, Pearce EL, Kass DA
medRxiv : the preprint server for health sciences 2023 Oct 2;

Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway.

He Y, Wang Y, Jia X, Li Y, Yang Y, Pan L, Zhao R, Han Y, Wang F, Guan X, Hou T
International immunopharmacology 2023 Jun;119:110192

MIR34A modulates lens epithelial cell apoptosis and cataract development via the HK1/caspase 3 signaling pathway.

Feng L, Wei Y, Sun Y, Zhou L, Bi S, Chen W, Xiang W
Aging 2023 Jul 6;15(13):6331-6345

Glycolytic Plasticity of Metastatic Lung Cancer Captured by Noninvasive (18)F-FDG PET/CT and Serum (1)H-NMR Analysis: An Orthotopic Murine Model Study.

Chung YH, Hung TH, Yu CF, Tsai CK, Weng CC, Jhang F, Chen FH, Lin G
Metabolites 2023 Jan 9;13(1)

The Effects of Prenatal Dexamethasone Exposure on Brain Metabolic Homeostasis in Adulthood: Implications for Depression.

Głombik K, Kukla-Bartoszek M, Curzytek K, Detka J, Basta-Kaim A, Budziszewska B
International journal of molecular sciences 2023 Jan 6;24(2)

Culture impact on the transcriptomic programs of primary and iPSC-derived human alveolar type 2 cells.

Alysandratos KD, Garcia-de-Alba C, Yao C, Pessina P, Huang J, Villacorta-Martin C, Hix OT, Minakin K, Burgess CL, Bawa P, Murthy A, Konda B, Beers MF, Stripp BR, Kim CF, Kotton DN
JCI insight 2023 Jan 10;8(1)

Effects of propranolol on glucose metabolism in hemangioma-derived endothelial cells.

Yang K, Li X, Qiu T, Zhou J, Gong X, Lan Y, Ji Y
Biochemical pharmacology 2023 Dec;218:115922

Glucose-Induced Activation of mTORC1 is Associated with Hexokinase2 Binding to Sestrins in HEK293T Cells.

Roberson PA, Kincheloe GN, Welles JE, Xu D, Sam-Clarke M, MacLean PS, Lang CH, Jefferson LS, Kimball SR
The Journal of nutrition 2023 Apr;153(4):988-998

Development and validation of a novel hypoxia-related signature for prognostic and immunogenic evaluation in head and neck squamous cell carcinoma.

Li SR, Man QW, Liu B
Frontiers in oncology 2022;12:943945

Engineering of thick human functional myocardium via static stretching and electrical stimulation.

Pretorius D, Kahn-Krell AM, LaBarge WC, Lou X, Zhang J
iScience 2022 Mar 18;25(3):103824

Long non-coding RNA CTSLP8 mediates ovarian cancer progression and chemotherapy resistance by modulating cellular glycolysis and regulating c-Myc expression through PKM2.

Li X, Zhang Y, Wang X, Lin F, Cheng X, Wang Z, Wang X
Cell biology and toxicology 2022 Dec;38(6):1027-1045

GM3 synthase deficiency increases brain glucose metabolism in mice.

Bharathi SS, Zhang BB, Paul E, Zhang Y, Schmidt AV, Fowler B, Wu Y, Tiemeyer M, Inamori KI, Inokuchi JI, Goetzman ES
Molecular genetics and metabolism 2022 Dec;137(4):342-348

GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.

Ahat E, Bui S, Zhang J, da Veiga Leprevost F, Sharkey L, Reid W, Nesvizhskii AI, Paulson HL, Wang Y
The Journal of biological chemistry 2022 Aug;298(8):102219

CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3.

Wang L, Lin Y, Zhou X, Chen Y, Li X, Luo W, Zhou Y, Cai L
Cancer letters 2022 Apr 28;532:215586

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

Chen X, Sun N, Li R, Sang X, Li X, Zhao J, Han J, Yang J, Ikezoe T
International journal of biological sciences 2021;17(5):1263-1276

HIF-1 regulates energy metabolism of the Tibetan chicken brain during embryo development under hypoxia.

Tang Q, Xu Q, Ding C, Zhang H, Ling Y, Wu C, Fang M
American journal of physiology. Regulatory, integrative and comparative physiology 2021 May 1;320(5):R704-R713

Androgen Receptor Stimulates Hexokinase 2 and Induces Glycolysis by PKA/CREB Signaling in Hepatocellular Carcinoma.

Sun RF, Zhao CY, Chen S, Yu W, Zhou MM, Gao CR
Digestive diseases and sciences 2021 Mar;66(3):802-813

SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.

Shangguan X, He J, Ma Z, Zhang W, Ji Y, Shen K, Yue Z, Li W, Xin Z, Zheng Q, Cao Y, Pan J, Dong B, Cheng J, Wang Q, Xue W
Nature communications 2021 Mar 22;12(1):1812

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Shi W, Fu Y, Wang Y
Oncology letters 2021 Jul;22(1):527

NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Gu M, Zhou X, Sohn JH, Zhu L, Jie Z, Yang JY, Zheng X, Xie X, Yang J, Shi Y, Brightbill HD, Kim JB, Wang J, Cheng X, Sun SC
Nature immunology 2021 Feb;22(2):193-204

JAK2(V617F) Mutation Promoted IL-6 Production and Glycolysis via Mediating PKM1 Stabilization in Macrophages.

Li R, Sun N, Chen X, Li X, Zhao J, Cheng W, Hua H, Fukatsu M, Mori H, Takahashi H, Ohkawara H, Fukami M, Okamoto M, Hamazaki Y, Zheng K, Yang J, Ikezoe T
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Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Zhang X, Wang R, Hu D, Sun X, Fujioka H, Lundberg K, Chan ER, Wang Q, Xu R, Flanagan ME, Pieper AA, Qi X
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He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

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Setiawan R, Priyadarshana C, Tajima A, Travis AJ, Asano A
Reproduction, fertility, and development 2020 Apr;32(7):697-705

Deoxyelephantopin suppresses hepatic stellate cells activation associated with inhibition of aerobic glycolysis via hedgehog pathway.

Gao W, Sun J, Wang F, Lu Y, Wen C, Bian Q, Wu H
Biochemical and biophysical research communications 2019 Sep 3;516(4):1222-1228

Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells.

Guo X, Wu S, Li N, Lin Q, Liu L, Liang H, Niu Y, Huang Z, Fu X
Biomolecules 2019 Sep 2;9(9)

The combination of metformin and 2-deoxyglucose significantly inhibits cyst formation in miniature pigs with polycystic kidney disease.

Lian X, Wu X, Li Z, Zhang Y, Song K, Cai G, Li Q, Lin S, Chen X, Bai XY
British journal of pharmacology 2019 Mar;176(5):711-724

Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.

Song G, Lu Q, Fan H, Zhang X, Ge L, Tian R, Wang S, Feng T, Pan J, Feng J, Xiao Y, Yi X, Ren N, Wang L
Arthritis research & therapy 2019 Apr 3;21(1):87

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Paulo E, Wu D, Wang Y, Zhang Y, Wu Y, Swaney DL, Soucheray M, Jimenez-Morales D, Chawla A, Krogan NJ, Wang B
Scientific reports 2018 Jul 20;8(1):11001

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Lian X, Zhao J, Wu X, Zhang Y, Li Q, Lin S, Bai XY, Chen X
Biochemical and biophysical research communications 2017 Jun 24;488(2):374-381

Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.

Song H, Yoon SP, Kim J
Anatomy & cell biology 2016 Jun;49(2):79-87

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human brain tissue were subjected to SDS PAGE followed by western blot with 19662-1-AP(HK1 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human testis using 19662-1-AP(HK1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Hexokinase 1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human Hexokinase 1. This antibody recognizes human,mouse,rat antigen. The Hexokinase 1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 19662-1-AP.pdf

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