MAB1101

antibody from Abnova Corporation

Targeting: HK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1101

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1101, RRID:AB_1575921

Product name

HK1 monoclonal antibody, clone 4D7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against full length recombinant HK1.

Isotype

IgG

Antibody clone number

4D7

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function.

Wilson JE
The Journal of experimental biology 2003 Jun;206(Pt 12):2049-57

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Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization.

Furuta H, Nishi S, Le Beau MM, Fernald AA, Yano H, Bell GI
Genomics 1996 Aug 15;36(1):206-9

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Studies on the mechanism of orthophosphate regulation of bovine brain hexokinase.

Ellison WR, Lueck JD, Fromm HJ
The Journal of biological chemistry 1975 Mar 10;250(5):1864-71

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates of HepG2 (30 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with HK1 monoclonal antibody , clone 4D7 (1 : 1000) (Cat # MAB1101). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.