PAB4009

antibody from Abnova Corporation

Targeting: HK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4009

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4009, RRID:AB_1575922

Product name

HK1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of HK1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

HK Utrecht: missense mutation in the active site of human hexokinase associated with hexokinase deficiency and severe nonspherocytic hemolytic anemia.

van Wijk R, Rijksen G, Huizinga EG, Nieuwenhuis HK, van Solinge WW
Blood 2003 Jan 1;101(1):345-7

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Gene expression and biological significance of hexokinase in erythroid cells.

Murakami K, Kanno H, Tancabelic J, Fujii H
Acta haematologica 2002;108(4):204-9

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Human HKR isozyme: organization of the hexokinase I gene, the erythroid-specific promoter, and transcription initiation site.

Murakami K, Kanno H, Miwa S, Piomelli S
Molecular genetics and metabolism 1999 Jun;67(2):118-30

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HK1 polyclonal antibody (Cat # PAB4009) in A-375 cell lysate. HK1 (arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with HK1 polyclonal antibody (Cat # PAB4009) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)