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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-15675 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HK1 Monoclonal Antibody (7A7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15675 targets HK1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Rat samples. The MA5-15675 immunogen is purified recombinant fragment of human HK1 expressed in E. Coli. MA5-15675 detects HK1 which has a predicted molecular weight of approximately 102kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7A7
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Galactose protects against cell damage in mouse models of acute pancreatitis.
Peng S, Gerasimenko JV, Tsugorka TM, Gryshchenko O, Samarasinghe S, Petersen OH, Gerasimenko OV
The Journal of clinical investigation 2018 Aug 31;128(9):3769-3778
The Journal of clinical investigation 2018 Aug 31;128(9):3769-3778
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using HK1 monoclonal antibody (Product # MA5-15675) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 HK activity is significantly inhibited in vitro by POA and BA. ( A ) HK1 activity is reduced significantly by 0.1 mM POA ( n = 6, P < 0.0001), but not changed significantly by 0.05% BA ( n = 4, P > 0.13) as compared with control ( n = 6). ( B ) HK2 activity is reduced significantly by 0.1 mM POA ( n = 9, P < 0.0001), but not affected by 0.05% BA ( n = 4, P > 0.3) as compared with control ( n = 13). ( C ) HK4 activity is reduced significantly by 0.05% BA ( n = 8, P < 0.0001), but not affected by 0.1 mM POA ( n = 4, P > 0.8) as compared with control ( n = 6). ( D ) Western blot analysis of the expression levels of HK1, HK2, and HK4 in PACs (representative case, repeated 3 times with similar results). *** P < 0.001, 1-way ANOVA.
