MA5-15680

antibody from Invitrogen Antibodies

Targeting: HK1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15680

Provider

Invitrogen Antibodies

Product name

HK1 Monoclonal Antibody (3A10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15680 targets HK1 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, mouse, and Rat samples. The MA5-15680 immunogen is purified recombinant fragment of human HK1 expressed in E. Coli. MA5-15680 detects HK1 which has a predicted molecular weight of approximately 120kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3A10

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Traumatic stress history interacts with sex and chronic peripheral inflammation to alter mitochondrial function of synaptosomes.

Shaw GA, Hyer MM, Targett I, Council KR, Dyer SK, Turkson S, Burns CM, Neigh GN
Brain, behavior, and immunity 2020 Aug;88:203-219

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), PC-12 (Lane 2), A-431 (Lane 3), U-87 MG (Lane 4), MCF7 (Lane 5), K-562 (Lane 6), Panc-1 (Lane 7), Caki-1 (Lane 8) and tissue extract (30 µg lysate) of Mouse Brain (Lane 9). The blot was probed with Anti-HK1 Monoclonal Antibody (Product # MA5-15680, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 102 kDa band corresponding to HK1 was observed across the cell lines and tissue extract tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), PC-12 (Lane 2), A-431 (Lane 3), U-87 MG (Lane 4), MCF7 (Lane 5), K-562 (Lane 6), Panc-1 (Lane 7), Caki-1 (Lane 8) and tissue extract (30 µg lysate) of Mouse Brain (Lane 9). The blot was probed with Anti-HK1 Monoclonal Antibody (Product # MA5-15680, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 102 kDa band corresponding to HK1 was observed across the cell lines and tissue extract tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HK1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HK1 Monoclonal Antibody (3A10) (Product # MA5-15680) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using HK1 monoclonal antibody (Product # MA5-15680) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 monoclonal antibody (Product # MA5-15680) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of K562 cells using HK1 monoclonal antibody (Product # MA5-15680) (green) and negative control (purple).