Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-22193 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Osteosarcoma cell-derived exosomes affect tumor microenvironment by specific packaging of microRNAs.
Raimondi L, De Luca A, Gallo A, Costa V, Russelli G, Cuscino N, Manno M, Raccosta S, Carina V, Bellavia D, Conigliaro A, Alessandro R, Fini M, Conaldi PG, Giavaresi G
Carcinogenesis 2020 Jul 10;41(5):666-677
Carcinogenesis 2020 Jul 10;41(5):666-677
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mAb against human Alkaline Phosphatase, Tissue Non-Specific (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of ovarian cancer tissues using Alkaline Phosphatase, Tissue Non-Specific mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of MCF-7 cells using Alkaline Phosphatase, Tissue Non-Specific mouse mAb (green) and negative control (red).