Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB29092 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Alkaline Phosphatase/ALPL Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Alkaline Phosphatase/ALPL in direct ELISA and Western Blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Alkaline Phosphatase/ALPL by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Alkaline Phosphatase/ALPL in Human Liver. Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.