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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- NB600-540 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-540, RRID:AB_2305446
- Product name
- Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver/ Bone/ Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells. This antibody does not cross react with other isozymes of AP.
- Reactivity
- Human, Feline, Porcine, Rabbit
- Host
- Mouse
- Antigen sequence
Liver/Bone/Kidney Alkaline Phosphat
ase- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment.
A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.
Wu D, Wang Z, Wang J, Geng Y, Zhang Z, Li Y, Li Q, Zheng Z, Cao Y, Zhang ZY
Stem cell research & therapy 2018 Nov 28;9(1):331
Stem cell research & therapy 2018 Nov 28;9(1):331
A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.
Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L
Stem cells translational medicine 2015 Apr;4(4):320-32
Stem cells translational medicine 2015 Apr;4(4):320-32
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).
