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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-24845 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALPL Recombinant Rabbit Monoclonal Antibody (3G2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3G2
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL.
Weng Y, Wang H, Wu D, Xu S, Chen X, Huang J, Feng Y, Li L, Wang Z
Cell research 2022 Sep;32(9):814-830
Cell research 2022 Sep;32(9):814-830
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ALPL in SW480 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS and incubated with ALPL monoclonal antibody (Product # MA5-24845).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ALPL in HepG2 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ALPL monoclonal antibody (Product # MA5-24845).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ALPL in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ALPL monoclonal antibody (Product # MA5-24845).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ALPL in HeLa cells (blue) and compared with an unlabeled control (cells without incubation with primary antibody; red). Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) at a dilution of 1:50 followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.
