Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00000249-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000249-M01, RRID:AB_536746
- Product name
- Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ALPL - alkaline phosphatase, liver/bone/kidney
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - ALPL monoclonal antibody (M01), clone 4H1 Western Blot analysis of ALPL expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.