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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- A01008-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Alkaline phosphatase/ALPL Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Alkaline phosphatase/ALPL detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling.
Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.
Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats.
Shan C, Wu Z, Xia Y, Ji X, Zhang W, Peng X, Zhao J
International immunopharmacology 2024 Jun 30;135:112299
International immunopharmacology 2024 Jun 30;135:112299
Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.
Jiang T, Zhao J, Yu S, Mao Z, Gao C, Zhu Y, Mao C, Zheng L
Biomaterials 2019 Jan;188:130-143
Biomaterials 2019 Jan;188:130-143
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.
Zhu L, Ma J, Mu R, Zhu R, Chen F, Wei X, Shi X, Zang S, Jin L
Life sciences 2018 Jun 1;202:175-181
Life sciences 2018 Jun 1;202:175-181
Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats.
Yu CH, Dai XY, Chen Q, Zang JN, Deng LL, Liu YH, Ying HZ
Carbohydrate polymers 2013 Apr 15;94(1):56-62
Carbohydrate polymers 2013 Apr 15;94(1):56-62
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Alkaline phosphatase/ALPL using anti-Alkaline phosphatase/ALPL antibody (A01008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alkaline phosphatase/ALPL antigen affinity purified polyclonal antibody (Catalog # A01008-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alkaline phosphatase/ALPL at approximately 80KD. The expected band size for Alkaline phosphatase/ALPL is at 57KD.
- Additional image
