Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB29091 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Alkaline Phosphatase/ALPL Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Alkaline Phosphatase/ALPL in direct ELISAs. In direct ELISAs, 80-100% cross-reactivity with recombinant mouse Alkaline Phosphatase/ALPL is observed and no cross-reactivity with recombinant human (rh) Alkaline Phosphatase/ALPP or rhAlkaline Phosphatase/ALPI is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P05186
- Isotype
- IgG
- Antibody clone number
- 388828
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
Central role of pyrophosphate in acellular cementum formation.
Enzyme replacement prevents enamel defects in hypophosphatasia mice.
Onishi K, Tonge PD, Nagy A, Zandstra PW
Stem cell reports 2014 Jul 8;3(1):156-68
Stem cell reports 2014 Jul 8;3(1):156-68
Central role of pyrophosphate in acellular cementum formation.
Foster BL, Nagatomo KJ, Nociti FH Jr, Fong H, Dunn D, Tran AB, Wang W, Narisawa S, Millán JL, Somerman MJ
PloS one 2012;7(6):e38393
PloS one 2012;7(6):e38393
Enzyme replacement prevents enamel defects in hypophosphatasia mice.
Yadav MC, de Oliveira RC, Foster BL, Fong H, Cory E, Narisawa S, Sah RL, Somerman M, Whyte MP, Millán JL
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Aug;27(8):1722-34
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Aug;27(8):1722-34
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Alkaline Phosphatase/ALPL in BG01V Human Embryonic Stem Cells. Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.