R1502P

antibody from OriGene

Targeting: RNF2 BAP-1, BAP1, DING, HIPI3, RING1B, RING2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1502P

Provider

OriGene

Product name

RNF2 (189-201) goat polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

RNF2 (189-201) goat polyclonal antibody, Aff - Purified

Host

Goat

Conjugate

Unconjugated

Epitope

RNF2

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.2 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-RING1B shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

RNF2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 2.5 ug/ml followed by biotinylated anti-Goat IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

Validation comment

IHC