PA5-28453

antibody from Invitrogen Antibodies

Targeting: RNF2 BAP-1, BAP1, DING, HIPI3, RING1B, RING2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28453

Provider

Invitrogen Antibodies

Product name

RNF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: H1299, HepG2, Molt-4, Raji.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RNF2 Polyclonal Antibody (Product # PA5-28453). Sample (30 µg of whole cell lysate). A: MOLT4SDS PAGE. Z. 12% SDS PAGE. RNF2 Polyclonal Antibody (Product # PA5-28453) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-RNF2 Polyclonal Antibody (Product # PA5-28453) and a 38 kDa band corresponding to RNF2 was observed across all the cell lines tested except in Raji which is reported be low to negative for the same. Modified whole cell extracts (1% SDS) (30 µg lysate) of K-562 (Lane 1), HCT-116 (Lane 2), Jurkat (Lane 3), MOLT-4 (Lane 4) and Raji (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1.25 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).