Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R1502P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1502P, RRID:AB_1007052
- Product name
- anti RNF2 (189-201)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding aa 189-201 of Human RING1B protein
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 1.2 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-RING1B shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- R1502P RNF2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 2.5 µg/ml followed by biotinylated anti-Goat IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.