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PA5-22173

antibody from Invitrogen Antibodies

Targeting: ULK2 ATG1B, KIAA0623, Unc51.2

Provider product page for PA5-22173

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [5]
Immunocytochemistry [2]
Immunohistochemistry [1]

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Validation data

Product number: PA5-22173 - Provider product page
Provider: Invitrogen Antibodies
Product name: ULK2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1, NIH-3T3.
Concentration: 0.33 mg/mL

ULK2 protein structure - PA5-22173 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ULK2 using 30 µg of A) NT2D1 and B) PC-3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ULK2 polyclonal antibody (Product # PA5-22173) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ULK2 using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ULK2 polyclonal antibody (Product # PA5-22173) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ULK2 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ULK2 polyclonal antibody (Product # PA5-22173) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ULK2 was performed by separating 30 µg of whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ULK2 Polyclonal Antibody (Product # PA5-22173) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ULK2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ULK2 Polyclonal Antibody (Product # PA5-22173) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.