Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3288 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3288, RRID:AB_1577656
- Product name
- NEK7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NEK7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J
The Journal of biological chemistry 2003 Sep 12;278(37):34897-909
The Journal of biological chemistry 2003 Sep 12;278(37):34897-909
Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3.
Kimura M, Okano Y
Cytogenetics and cell genetics 2001;94(1-2):33-8
Cytogenetics and cell genetics 2001;94(1-2):33-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NEK7 (arrow) using NEK7 polyclonal antibody (Cat # PAB3288).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK7 polyclonal antibody (Cat # PAB3288) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)