Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-47008 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK7 Recombinant Rabbit Monoclonal Antibody (HL1349)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Store as concentrated solution. Centrifuge briefly prior to opening vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1349
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEK7 in wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with NEK7 Monoclonal antibody (Product # MA5-47008) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEK7 in Various whole cell extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with NEK7 Monoclonal antibody (Product # MA5-47008) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NEK7 in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 stained by NEK7 monoclonal antibody (Product # MA5-47008) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NEK7 in paraffin-embedded mouse stomach. Samples were incubated in NEK7 Monoclonal antibody (Product # MA5-47008). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NEK7 in paraffin-embedded mouse stomach. Samples were incubated in NEK7 Monoclonal antibody (Product # MA5-47008). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
