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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00023528-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023528-M01, RRID:AB_2217157
- Product name
- Mouse Monoclonal ZNF281 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ZNF281 - zinc finger protein 281
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 over-expressed 293 cell line, cotransfected with ZNF281 Validated Chimera RNAi ( Cat # H00023528-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF281 monoclonal antibody (M01), clone 4F7 (Cat # H00023528-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 expression in transfected 293T cell line by ZNF281 monoclonal antibody (M01), clone 4F7.Lane 1: ZNF281 transfected lysate(96.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ZNF281 Antibody (4F7) [H00023528-M01] - Detection limit for recombinant GST tagged ZNF281 is approximately 0.03ng/ml as a capture antibody.
