Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21121 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF281 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A-20 cell lysate. PA5-21121 can be used with blocking peptide PEP-1235.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- 4°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A20 cells using a ZFP281 polyclonal antibody (Product # PA5-21121) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ZFP291 in A20 cells with ZNF281 Polyclonal Antibody (Product # PA5-21121) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZFP281 (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a ZFP281 polyclonal antibody (Product # PA5-21121) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-rabbit IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ZFP291 in A20 cells with ZNF281 Polyclonal Antibody (Product # PA5-21121) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ZFP291 in A20 cells with ZNF281 Polyclonal Antibody (Product # PA5-21121) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZFP281 (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a ZFP281 polyclonal antibody (Product # PA5-21121) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-rabbit IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306).
