32-5700

antibody from Invitrogen Antibodies

Targeting: PKP1 B6P

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

32-5700

Provider

Invitrogen Antibodies

Product name

PKP1 Monoclonal Antibody (10B2)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10B2

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Analysis of buccal mucosa as a prognostic tool in children with arrhythmogenic cardiomyopathy.

Bueno-Beti C, Field E, Tsatsopoulou A, Perry G, Sheppard MN, Behr ER, Saffitz JE, Kaski JP, Asimaki A
Progress in pediatric cardiology 2022 Mar;64:None

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Homozygous splice site mutations in PKP1 result in loss of epidermal plakophilin 1 expression and underlie ectodermal dysplasia/skin fragility syndrome in two consanguineous families.

Sprecher E, Molho-Pessach V, Ingber A, Sagi E, Indelman M, Bergman R
The Journal of investigative dermatology 2004 Mar;122(3):647-51

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Defining desmosomal plakophilin-3 interactions.

Bonné S, Gilbert B, Hatzfeld M, Chen X, Green KJ, van Roy F
The Journal of cell biology 2003 Apr 28;161(2):403-16

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Desmoglein 4 in hair follicle differentiation and epidermal adhesion: evidence from inherited hypotrichosis and acquired pemphigus vulgaris.

Kljuic A, Bazzi H, Sundberg JP, Martinez-Mir A, O'Shaughnessy R, Mahoney MG, Levy M, Montagutelli X, Ahmad W, Aita VM, Gordon D, Uitto J, Whiting D, Ott J, Fischer S, Gilliam TC, Jahoda CA, Morris RJ, Panteleyev AA, Nguyen VT, Christiano AM
Cell 2003 Apr 18;113(2):249-60

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Desmoglein 4 in hair follicle differentiation and epidermal adhesion: evidence from inherited hypotrichosis and acquired pemphigus vulgaris.

Kljuic A, Bazzi H, Sundberg JP, Martinez-Mir A, O'Shaughnessy R, Mahoney MG, Levy M, Montagutelli X, Ahmad W, Aita VM, Gordon D, Uitto J, Whiting D, Ott J, Fischer S, Gilliam TC, Jahoda CA, Morris RJ, Panteleyev AA, Nguyen VT, Christiano AM
Cell 2003 Apr 18;113(2):249-60

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Plakophilin (PKP1) was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PKP1 (10B2) Mouse Monoclonal Antibody (Product # 32-5700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Immunodetection of PKPs using different mouse mAbs. PKP3, detected with mAb 23E3/4 (a and b), 12B11F8 (c), or PKP2 (d) show a similar expression pattern along cell-cell contacts in methanol-fixed HaCaT (a) and HCT8/E8 (b-d) cells. Larger magnifications display the punctate localization of these proteins along cell-cell contacts (a'-d'). Bar, 10 mum. (e) Western blot detection of PKP3 (lane 1, mAb 12B11F8; lane 2, 23E3/4), PKP2 (lane 3), and PKP1 (lane 4) in HaCaT protein lysates. Equal amounts of protein were loaded in each lane.