AF6477

antibody from Novus Biologicals

Targeting: KDM5B CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF6477

Provider

Novus Biologicals

Product name

Sheep Polyclonal Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human KDM5B in Western blots.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Lysine (K)-specific Demethylase 5B/KDM5B by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 5B/KDM5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6477) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 5B/KDM5B at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.