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LearnH00010765-M02
antibody from Novus Biologicals
Targeting: KDM5B
CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010765-M02 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. JARID1B (1G10)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma.
Jumonji/ARID1 B (JARID1B) protein promotes breast tumor cell cycle progression through epigenetic repression of microRNA let-7e.
Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway.
JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.
Shigekawa Y, Hayami S, Ueno M, Miyamoto A, Suzaki N, Kawai M, Hirono S, Okada KI, Hamamoto R, Yamaue H
Oncotarget 2018 Sep 28;9(76):34320-34335
Oncotarget 2018 Sep 28;9(76):34320-34335
Jumonji/ARID1 B (JARID1B) protein promotes breast tumor cell cycle progression through epigenetic repression of microRNA let-7e.
Mitra D, Das PM, Huynh FC, Jones FE
The Journal of biological chemistry 2011 Nov 25;286(47):40531-5
The Journal of biological chemistry 2011 Nov 25;286(47):40531-5
Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway.
Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T, Field HI, Kelly JD, Neal DE, Yamaue H, Ponder BA, Nakamura Y, Hamamoto R
Molecular cancer 2010 Mar 13;9:59
Molecular cancer 2010 Mar 13;9:59
JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.
Xiang Y, Zhu Z, Han G, Ye X, Xu B, Peng Z, Ma Y, Yu Y, Lin H, Chen AP, Chen CD
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 4;104(49):19226-31
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 4;104(49):19226-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of JARID1B expression in Hela NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - KDM5B monoclonal antibody (M02), clone 1G10. Analysis of KDM5B expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (1G10) [H00010765-M02] - Analysis of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
